Halicephalobus Infection in a Fatal Case of Encephalomyelitis

نویسندگان

  • Cynthia S. Goldsmith
  • Mark L. Eberhard
  • Julu Bhatnagar
  • Clifton Drew
چکیده

A female with encephalitis died 29 days after hospitalization. Histological examination of the brain revealed several nematodes, mostly larva, although a later stage larva/early adult worm was found and was morphologically consistent as being in the genus Halicephalobus. Halicephalobus specific and cephalob nematode specific PCR assays were performed on DNA extracted from unfixed frozen tissues, and sequence analysis of positive amplicons showed 99% identity with Halicephalobus spp. A formalin-fixed paraffin-embedded section was processed for EM, and EM examination was consistent with the finding of a nematode. The worm was up to 190 μm in length and up to 20 μm in diameter, with a long intestine, muscle fibers, an anus, and developing eggs and germinal cells (Figures 1-4). There was an area of vacant space between the cuticle of the worm and the parenchyma of the brain.

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تاریخ انتشار 2014